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The Anti‐Inflammatory Properties of Rhododendron molle Leaf Extract in LPS‐Induced RAW264.7
Author(s) -
Zong Luye,
Zhang Jin,
Dai Liangfang,
Liu Jian,
Yang Yan,
Xie Jiankun,
Luo Xiangdong
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202000477
Subject(s) - lipopolysaccharide , chemistry , tumor necrosis factor alpha , inflammation , griess test , enzyme , anti inflammatory , proinflammatory cytokine , microbiology and biotechnology , pharmacology , biochemistry , immunology , nitrite , biology , organic chemistry , nitrate
Rhododendron molle G.Don is a well‐known traditional medicine which has been used to treat rheumatic inflammation. In this study, an inflammatory model of lipopolysaccharide (LPS)‐stimulated RAW264.7 cells was established to analyze the anti‐inflammatory effect and potential mechanism of the methanol extract of R. molle leaves (RLE). The production of NO and the expression of tumor necrosis factor by LPS were detected by Griess reaction and enzyme linked immunosorbent assay (ELISA). The mRNA expression of TNF‐α , IL‐1β , IL‐6 , COX‐2 and iNOS was measured by qRT‐PCR assay. Griess and qRT‐PCR showed that the RLE could significantly concentration‐dependently inhibit NO production and the expression of many pro‐inflammatory cytokines and inflammatory‐related enzymes. Scanning electron microscope (SEM) analysis indicated that RLE could inhibit LPS‐stimulated RAW264.7 macrophages activation. The protein level of TNF‐ α and IL‐1 β were decreased over 50 % at 100 μg/ml of RLE, as detected by ELISA. These results indicated that RLE had strong anti‐inflammatory and immunomodulatory activity.