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Protective Antioxidant Effects of Amentoflavone and Total Flavonoids from Hedyotis diffusa on H 2 O 2 ‐Induced HL‐O2 Cells through ASK1/p38 MAPK Pathway
Author(s) -
Li YunLan,
Chen Xi,
Niu SiQing,
Zhou HongYan,
Li QingShan
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202000251
Subject(s) - amentoflavone , chemistry , p38 mitogen activated protein kinases , antioxidant , reactive oxygen species , apoptosis , mapk/erk pathway , hepatoprotection , biochemistry , pharmacology , kinase , enzyme , glutathione , biology
In this study, total flavonoids and total triterpenoid acid were extracted with ethyl acetate from Hedyotis diffusa Willd, and hepatoprotective activities of them and five compounds from total flavonoids against H 2 O 2 induced hepatocyte damage on HL‐02 cells were determined. In particular, amentoflavone and total flavonoids had influence on the leakage of ALT, AST, LDH, the activities of SOD and the content of MDA. They effectively reduced the loss of MMP, the release of Cyt C, and then inhibited activation of caspase‐3/caspase‐9 cascade in hepatotoxic cells. The contents of ROS were significantly reduced to inhibit p38 in amentoflavone and flavonoids groups which decreased ASK1 and p‐p38 levels through increasing thioredoxin Trx1 and reductase TrxR1. These results suggesting that the antioxidant protection of amentoflavone and flavonoids might be reducing ROS to inhibit the H 2 O 2 ‐induced upstream of pathway via increasing levels of Trx1 and TrxR1, which were pivotal in blocking the down streaming effectors of ASK1/p38 MAPK pathway and alleviating hepatotoxicity.