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Development and Validation of HPLC Method for Determination of Sodium Copper Chlorophyllin – A Food Colorant and Its Application in Pharmacokinetic Study
Author(s) -
Laddha Ankit P.,
Nalawade Vivek V.,
Gharpure Milind,
Kulkarni Yogesh A.
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202000223
Subject(s) - chemistry , bioanalysis , ammonium acetate , chromatography , sodium acetate , chlorophyllin , copper , pharmacokinetics , particle size , sodium , high performance liquid chromatography , methanol , organic chemistry , medicine , chlorophyll
A simple, accurate, and precise bioanalytical method was developed and validated for the determination of pharmacokinetic parameters of sodium copper chlorophyllin, a USFDA approved food additive and colorant in rat plasma. The column used was Luna ® C 18 250×4.6 mm, 100 Å, having particle size 4.5 μm, and the mobile phase used was methanol (MeOH), and 10 mM ammonium acetate buffer in the ratio of 90 : 10, the flow rate was 1 ml/min, and the injection volume of 20 μL. The retention time of sodium copper chlorophyllin was obtained at 9 min. The method was found to be linear at the range of 0.50–8.00 μg mL −1 .