Development and Validation of HPLC Method for Determination of Sodium Copper Chlorophyllin – A Food Colorant and Its Application in Pharmacokinetic Study | Zendy

Laddha Ankit P. | Zendy; Nalawade Vivek V. | Zendy; Gharpure Milind | Zendy; Kulkarni Yogesh A. | Zendy

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Development and Validation of HPLC Method for Determination of Sodium Copper Chlorophyllin – A Food Colorant and Its Application in Pharmacokinetic Study

Author(s) -

Laddha Ankit P.,

Nalawade Vivek V.,

Gharpure Milind,

Kulkarni Yogesh A.

Publication year - 2020

Publication title -

chemistry and biodiversity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.427

H-Index - 70

eISSN - 1612-1880

pISSN - 1612-1872

DOI - 10.1002/cbdv.202000223

Subject(s) - chemistry , bioanalysis , ammonium acetate , chromatography , sodium acetate , chlorophyllin , copper , pharmacokinetics , particle size , sodium , high performance liquid chromatography , methanol , organic chemistry , medicine , chlorophyll

A simple, accurate, and precise bioanalytical method was developed and validated for the determination of pharmacokinetic parameters of sodium copper chlorophyllin, a USFDA approved food additive and colorant in rat plasma. The column used was Luna ® C 18 250×4.6 mm, 100 Å, having particle size 4.5 μm, and the mobile phase used was methanol (MeOH), and 10 mM ammonium acetate buffer in the ratio of 90 : 10, the flow rate was 1 ml/min, and the injection volume of 20 μL. The retention time of sodium copper chlorophyllin was obtained at 9 min. The method was found to be linear at the range of 0.50–8.00 μg mL −1 .

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