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A m 6 A Sensing Method by Its Impact on the Stability of RNA Double Helix
Author(s) -
Yang Shixi,
Wu Fan,
Peng Shuang,
Wang Fang,
Chen Yi,
Yuan Yushu,
Weng Xiaocheng,
Zhou Xiang
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.202000050
Subject(s) - rna , chemistry , rna silencing , epigenetics , computational biology , biophysics , rna interference , biochemistry , gene , biology
N 6 ‐Methyladenosine (m 6 A) is one of the most important RNA modifications in epigenetics. The development of detection method for m 6 A is limited by its abundance and structure. Although it has been previously reported that its presence has an impact on the complementary pairing of RNA, few assays have been developed using this finding. We used this discovery and designed a detection method based on Cas13a system, which has different fluorescence signals for target RNAs containing m 6 A modification and target RNAs without m 6 A modification. We verified the fact that the presence of m 6 A could cause the instability of dsRNA using the Cas13a system and provided a new direction and strategy for the development of m 6 A detection methods in the future.