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Brassica rapa Polysaccharides Ameliorate CCl 4 ‐Induced Acute Liver Injury in Mice through Inhibiting Inflammatory Apoptotic Response and Oxidative Stress
Author(s) -
Li Qiuyue,
Yang Hailing,
Wang Wenxiang,
Li Ning,
Zou Xuemei,
Li Yangxin,
Fan Gang,
Zhang Yi,
Kuang Tingting
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201900534
Subject(s) - chemistry , carbon tetrachloride , liver injury , pharmacology , ccl4 , oxidative stress , western blot , brassica rapa , apoptosis , glutathione , hepatoprotection , biochemistry , medicine , enzyme , organic chemistry , gene
Brassica rapa L., also called NIUMA, is used empirically in Tibetan medicine for its antioxidant, anti‐inflammatory and antiradiation activities. This study explored the hepatoprotective effects of B. rapa polysaccharides (BRPs) on acute liver injury induced by carbon tetrachloride (CCl 4 ) in mice and the underlying mechanisms. Mice were treated with CCl 4 after the oral administration of BRPs (55, 110 and 220 mg/kg) or bifendate (100 mg/kg) for 7 days. Blood and liver samples of mice were collected for analysis after 24 h. The ALP, ALT and AST levels and the biological activities of SOD, MDA and GSH−Px were measured. Histopathological changes in the liver were determined through hematoxylin and eosin staining. Moreover, TNF‐ α , IL‐1 β and IL‐6 expression levels were detected by commercial reagent kits. Finally, Western blot analysis was used to check the relative expression levels of caspase‐3, p ‐JAK2 and p ‐STAT3. The BRP pre‐treatment significantly decreased the enzymatic activities of ALT, ALP and AST in the serum, markedly increased the activities of SOD and GSH−Px in the liver and reduced the MDA concentration in the liver. BRPs alleviated hepatocyte injury and markedly inhibited the expression of TNF‐ α , IL‐1 β and IL‐6, also downregulating the CCl 4 ‐induced hepatic tissue expression of caspase‐3. Furthermore, BRPs inhibited the JAK2/STAT3 signaling pathway in a dose‐dependent manner in the liver. This study demonstrated that BRPs exert hepatoprotective effect against the CCl 4 ‐induced liver injury via modulating the apoptotic and inflammatory responses and downregulating the JAK2/STAT3 signaling pathway. Therefore, B. rapa could be considered a hepatoprotective medicine.