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Reliable Methods for Analyses of Volatile Compounds of Copaifera Oleoresins Combining Headspace and Gas Chromatography
Author(s) -
Mangabeira da Silva Jonas Joaquim,
Pena Ribeiro Victor,
Lemos Marivane,
Miller Crotti Antônio Eduardo,
Rogez Hervé,
Kenupp Bastos Jairo
Publication year - 2020
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201900440
Subject(s) - chemistry , chromatography , gas chromatography , flame ionization detector , sesquiterpene , mass spectrometry , extraction (chemistry) , gas chromatography–mass spectrometry , oleoresin , organic chemistry
Abstract Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography‐mass spectrometry (HS‐SPME‐GC/MS) showed the best results for oleoresin qualitative analysis, setting the following extraction conditions: equilibrium time of 15 min, extraction time of 30 min, extraction temperature at 60 °C and constant stirring of 400 rpm. Sesquiterpenes α ‐copaene, β ‐elemene, β ‐caryophyllene and trans‐α ‐bergamotene were found in all investigated samples. Quantitative analysis by gas chromatography coupled with flame ionization detector (GC‐FID) measured the content of the four sesquiterpenes in all samples. Qualitative and quantitative results showed important differences between COR of distinct species and commercial products. Data regarding the volatile composition of C. oblongifolia and C. trapezifolia oleoresins were first presented in this study and two new analytical methods were reported for direct and fast qualitative and quantitative analysis of COR.