Premium
Biotransformation of Huperzine B by a Fungal Endophyte of Huperzia serrata
Author(s) -
Zhan ZhaJun,
Tian Ting,
Xu YiLian,
Yu HangFei,
Zhang CaiXue,
Zhang ZhiDong,
Tang QiYong,
Shan WeiGuang,
Ying YouMin
Publication year - 2019
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201900299
Subject(s) - biotransformation , huperzine a , endophyte , chemistry , biochemistry , enzyme , stereochemistry , botany , microbiology and biotechnology , biology , acetylcholinesterase
The biotransformation of huperzine B (hupB), one of the characteristic bioactive constituents of the medicinal plant Huperzia serrata , by a fungal endophyte of the host plant was studied. One new compound, 8 α ,15 α ‐epoxyhuperzine B ( 1 ), along with two known oxygenated hupB analogs, 16‐hydroxyhuperzine B ( 2 ) and carinatumin B ( 3 ), was isolated and identified. The structures of all the isolates were deduced by spectroscopic methods including NMR, MS, IR, and UV spectra. The known compounds 2 and 3 were obtained from a microbial source for the first time. To the best of our knowledge, it is the first report on the microbial transformation of hupB and would facilitate further structural modification of hupB by chemo‐enzymatic method. In the LPS‐induced neuro‐inflammation injury assay, 8 α ,15 α ‐epoxyhuperzine B ( 1 ) exhibited moderate neuroprotective activity by increasing the viability of U251 cell lines with an EC 50 of 40.1 n m .