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Anti‐Adhesion Activity of Tannins Isolated from the Mangrove Laguncularia racemosa
Author(s) -
Glasenapp Yvana,
Lucas Christina,
Wöltje Timo,
Fohrer Jörg,
Papenbrock Jutta
Publication year - 2019
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201800632
Subject(s) - candida albicans , chemistry , biofilm , adhesion , mangrove , escherichia coli , corpus albicans , microbiology and biotechnology , biochemistry , biology , bacteria , organic chemistry , genetics , gene , ecology
In the search of new compounds with biofilm‐inhibiting properties, mangroves with their richness of secondary metabolites can be a valuable resource. Crude methanolic leaf extracts from the mangrove Laguncularia racemosa enriched in phenolic substances cause a reduction in initial cell adhesion of Candida glabrata and Candida albicans , but not on Escherichia coli . LC/MS‐guided fractionation of the phenolic compounds resulted in 19 fractions, of which ten were analyzed for their bioactivity against cell adhesion. Effects on cell adhesion and planktonic growth of Escherichia coli , Candida glabrata and Candida albicans were measured in 96‐well microtiter plates in the presence of 0.2 mg ml −1 of the isolated fractions. Two fractions caused a reduction of cell adhesion of Candida albicans . These fractions containing bioactive compounds were analyzed by LC/MS and NMR spectroscopy. Casuarinin and digalloyl‐hexahydroxydiphenoyl‐glucose were identified in the active fractions, in addition to three signals of ellagitannins. These results indicate a specific mode of action of hydrolysable tannins against cell adhesion of Candida albicans , which needs to be further analyzed.