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Antimycotic Activity Potentiation of Allium sativum Extract and Silver Nanoparticles against Trichophyton rubrum
Author(s) -
RoblesMartínez Marissa,
González Juan Fernando Cárdenas,
PérezVázquez Francisco Javier,
MontejanoCarrizales Juan Martín,
Pérez Elías,
PatiñoHerrera Rosalba
Publication year - 2019
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201800525
Subject(s) - trichophyton rubrum , chemistry , allium sativum , silver nanoparticle , antifungal , nuclear chemistry , minimum inhibitory concentration , sativum , in vitro , microbiology and biotechnology , nanoparticle , biochemistry , botany , nanotechnology , biology , materials science
A natural and biocompatible extract of garlic as a support, decorated with silver nanoparticles, is a proposal to generate an effective antifungal agent against dermatophytes at low concentrations. Silver nanoparticles (AgNPs) with a diameter of 26±7 nm were synthesized and their antimycotic activity was examined against Trichophyton rubrum ( T. rubrum ), inhibiting 94 % of growth at a concentration of 0.08 mg ml −1 . Allium sativum (garlic) extract was also obtained ( As Ext), and its MIC was 0.04 mg ml −1 . To increase the antifungal capacity of those systems, As Ext was decorated with AgNPs, obtaining As Ext‐AgNPs. Using an As Ext concentration of 0.04 mg ml −1 in independent experiments with concentrations from 0.01 to 0.08 mg ml −1 of AgNPs, it was possible to inhibit T. rubrum at all AgNPs concentrations; it proves a synergistic effect between AgNPs and As Ext. Even if 1 % of the minimum inhibitory concentration of As Ext (0.0004 mg ml −1 ) is used, it was possible to inhibit T. rubrum at all concentrations of AgNPs, demonstrating the successful antimycotic activity potentiation when combining As Ext and AgNPs.