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Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck . Seeds
Author(s) -
Jakovljević Violeta D.,
Vrvić Miroslav M.,
Vrbničanin Sava,
SarićKrsmanović Marija
Publication year - 2018
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201800174
Subject(s) - chemistry , phytochemical , antioxidant , dpph , food science , chloroform , flavonoid , glycoside , phenol , biochemistry , organic chemistry
This work was conceptualized with the goal to investigate the phytochemical, free radical scavenging and antifungal profile of Cuscuta campestris Yunck . seeds. Total phenolics, amino acid and carbohydrate contents were evaluated in ethanolic, acetone and chloroform extract. Effective antioxidant activity was evaluated throughout seven antioxidant methods. The antifungal activity was assessed against eight fungal strains and Candida albicans . The results showed total phenol, flavonoid, flavonols and phenolic acids contents in amount of 1.51 – 6.35 mg GAE / mL , 78 – 425 μg RU / mL , 1.04 – 2.98 mg QU /g and 12.01 – 30.58 μg CAE / mL , respectively. The total amino acids and carbohydrates content ranged from 8.29 to 185.45 μg Gly/ mL and from 0.05 to 0.12 μg Glu/ mL . The ethanolic extract showed the best antioxidant activity in phosphomolybdenum, DPPH free radical scavenging, ferric reducing power and lipid peroxidation assays. The best activity in ferrous ion chelating and H 2 O 2 assays had the acetone extract, whereas the best hydroxyl radical scavenging activity was observed with the chloroform extract. The ethanolic extract at a concentration of 6 mg/ mL proved to be the most effective antimycotic, since it inhibited the growth of all tested fungi except Penicillium verrucosum . The obtained results indicate that C . campestris seeds could be attributed to a potential source of natural antioxidants in food and pharmaceutical products.