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UHPLC‐HRMS/MS Based Profiling of Algerian Lichens and Their Antimicrobial Activities
Author(s) -
Brakni Rafika,
Ali Ahmed Monia,
Burger Pauline,
Schwing Aurélie,
Michel Grégory,
Pomares Christelle,
Hasseine Lillia,
Boyer Laurent,
Fernandez Xavier,
Landreau Anne,
Michel Thomas
Publication year - 2018
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201800031
Subject(s) - lichen , chemistry , antimicrobial , aspergillus fumigatus , candida glabrata , candida albicans , electrospray ionization , antifungal , parmeliaceae , microbiology and biotechnology , mass spectrometry , chromatography , biology , biochemistry , botany , ascomycota , organic chemistry , gene
Lichens are complex symbiotic organisms able to produce a vast array of compounds. The Algerian lichen diversity has only prompted little interest even given the 1085 species listed. Herein, the chemodiversity of four Algerian lichens including Cladonia rangiformis , Ramalina farinaceae , R . fastigiata , and Roccella phycopsis was investigated. A dereplication strategy, using ultra high performance liquid chromatography‐high resolution‐electrospray ionization‐mass spectrometry (UHPLC‐HRMS/MS), was carried out for a comprehensive characterization of their substances including phenolics, depsides, depsidones, depsones, dibenzofurans, and aliphatic acids. Some known compounds were identified for the first time in some species. Additionally, the lichenic extracts were evaluated for their antifungal and antimicrobial activities on human pathogenic strains ( Candida albicans , C . glabrata , Aspergillus fumigatus , Staphylococcus aureus , and Escherichia coli ). Cyclohexane extracts were found particularly active against human pathogenic fungi with MIC 80 values ranging from 8 to 62.5 μg/mL, without cytotoxicity. This study highlights the therapeutic and prophylactic potential of lichenic extracts as antibacterial and antifungal agents.