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ent ‐Sauchinone as Potential Anticancer Agent Inhibiting Migration and Invasion of Human Liver Cancer Cells via Suppressing the STAT 3 Signaling Pathway
Author(s) -
Gong QiZhe,
Xiao Di,
Feng Feng,
Wen XiaoDong,
Qu Wei
Publication year - 2018
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201800024
Subject(s) - cell migration , viability assay , metastasis , cancer cell , chemistry , mtt assay , stat protein , cancer research , cell culture , cytotoxicity , cancer , signal transduction , cell , traditional medicine , biology , biochemistry , in vitro , medicine , stat3 , genetics
ent ‐Sauchinone, a lignan isolated from Saururus chinensis ( Lour .) Baill ., was reported that it could modulate the expression of signal transducer and activator of transcription 3 ( STAT 3). Since STAT 3 plays a key role in invasion, migration, and metastasis of cancer, we investigated whether ent ‐sauchinone could exert promising inhibitory effects on the invasion and migration of the metastatic human liver cancer cell line SMMC ‐7721 in the present study. ent ‐Sauchinone was extracted from dried herbs of Saururus chinensis ( Lour .) Baill . Human liver cancer cell lines SMMC ‐7721 and HCCLM 3 were used to test the effect of ent ‐sauchinone on cell viability. The IC 50 values and time‐dependent effect of ent ‐sauchinone were determined by MTT assay. Cell migration and invasion of SMMC ‐7721 were evaluated by the wound healing test and transwell assay respectively, the known anti‐metastasis agent curcumin was used as a positive control. Western blotting assay was used to investigate relevant molecular mechanisms of cell invasion and migration. Though ent ‐sauchinone didn't show high cytotoxicity, the wound healing assay and transwell migration assay revealed a profound impairment in the metastatic potential of SMMC ‐7721 cells due to down‐regulation of N‐cadherin, MMP ‐2, and MMP ‐9 proteins induced by inhibiting the phosphorylation of STAT 3. These findings suggest that ent ‐sauchinone could be used as a promising agent to treat cancer metastasis.