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Simultaneous Preparation and Comparison of the Osteogenic Effects of Epimedins A – C and Icariin from Epimedium brevicornu
Author(s) -
Guo Yani,
Wang Xiufeng,
Gao Jing
Publication year - 2018
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700578
Subject(s) - icariin , epimedium , chemistry , chromatography , extraction (chemistry) , fractionation , silica gel , traditional medicine , medicinal herbs , medicine , alternative medicine , herb , pathology
In this study, epimedins A‐, B‐, and C‐, and icariin‐rich extracts were simultaneously isolated from Epimedium brevicornu Maxim . through a convenient four‐stage process consisting of solvent extraction, macroporous resin column pre‐concentration, extraction fractionation, and reversed‐phase ( RP ) silica gel column chromatography. Single factor experiments and response surface methodology ( RSM ) were used to optimize the preparation conditions. In the final products, the purities of epimedins A – C and icariin were 23.04%, 64.50%, 54.92%, and 77.54%, respectively, which will lay a foundation for the further purification of epimedin monomers and full utilization of Epimedium resources. Meanwhile, the osteogenic effects of epimedins A – C were investigated for the first time and compared with that of icariin, which will provide guidance for the clinical applications of Epimedium in the treatment of osteoporosis.