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Melanogenesis‐Inhibitory and Cytotoxic Activities of Chemical Constituents from the Leaves of Sauropus androgynus L. Merr . (Euphorbiaceae)
Author(s) -
Zhang Jie,
Zhu WanFang,
Zhu WeiYuan,
Yang Pan Pan,
Xu Jian,
Manosroi Jiradej,
Kikuchi Takashi,
Abe Masahiko,
Akihisa Toshihiro,
Feng Feng
Publication year - 2018
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700486
Subject(s) - euphorbiaceae , chemistry , inhibitory postsynaptic potential , traditional medicine , botany , biology , medicine , neuroscience
A new steroid, 20‐hydroxyisofucosterol (stigmasta‐5,24(28)‐diene‐3 β ,20 β ‐diol) ( 7 ), along with six known compounds 1 – 6 were isolated from the Me OH extract of the leaves of Sauropus androgynus L. Merr . (Euphorbiaceae). The structure of new steroid was determined by HR ‐ APCI ‐ MS and various NMR techniques in combination with literature data. Subsequently, their anti‐inflammatory, cytotoxic activities against five human cell lines, as well as inhibitory activities against the α ‐ MSH induced melanogenesis on the B16 cell line were evaluated. As the results, steroid compounds, 6 and 7 exhibited moderate cytotoxic to HL 60, AZ 521, SKBR 3, and A549 tumor cell lines ( IC 50 26.9 – 45.1 μ m ) with high tumor selectivity for A549 relative to WI 38 cell lines ( SI 2.6 and 3.0, resp.). And, flavonoid compounds, 4 and 5 exhibited superior inhibitory activities against melanogenesis (67.0 – 94.7% melanin content), even with no or low toxicity to the cells (90.1 – 99.6% cell viability) at the concentrations from 10 to 100 μ m . Furthermore, Western blot analysis suggested that compound 5 could inhibit melanogenesis by suppressing the protein expressions of MITF , TRP ‐1, TRP ‐2, and tyrosinase.