Premium
Quick Identification of Piperidine Alkaloid from Roots of Grewia nervosa and Their Glucosidase Inhibitory Activity
Author(s) -
Meena Surya N.,
Majik Mahesh S.,
Ghadi Sanjeev C.,
Tilve Santosh G.
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700400
Subject(s) - chemistry , acarbose , sephadex , piperidine , chromatography , alkaloid , column chromatography , fractionation , high performance liquid chromatography , thin layer chromatography , silica gel , stereochemistry , enzyme , biochemistry
Grewia nervosa is a herbal plant used in traditional medicine for different purposes. Bioassay‐guided chemical fractionation of G. nervosa roots resulted in an identification of two known and one new compound, namely microgrewiapine A, homomicrogrewiapine, and N ‐methylmicrocosamine, respectively. Their structures were determined using combination of LC/HR‐MS, 1 H‐NMR, and IR spectral analyses and followed by comparison with those reported in the literature. The problematic separation of these alkaloids on traditional column chromatography (Silica gel, Octadecyl silane, Sephadex ) was resolved by using HPLC. Structurally similar compounds from the piperidine family have been characterized by using HR‐MS analysis in combination with NMR data of crude samples. The major constituent i.e. N ‐methylmicrocosamine isolated from the butanol fraction of methanol root extract (MRE) was found to possess the dose dependent α ‐glucosidase inhibition activity with an IC 50 value of 53.40 μ m . Furthermore, N ‐methylmicrocosamine showed maximum α ‐glucosidase inhibition of 97.48 ± 0.7% at 107.5 μ m , which is approximately 1.3 × 10 3 fold higher than the activity shown by acarbose (97.72% inhibition at 61.95 m m ), a standard anti‐diabetic drug available commercially. This work also reports the in vitro α ‐glucosidase inhibitory activity of the major alkaloids isolated from G. nervosa for the first time.