Antiviral Activity of Faramea hyacinthina and Faramea truncata Leaves on Dengue Virus Type‐2 and Their Major Compounds

The defatted fractions of the Faramea hyacinthina and F .  truncata (Rubiaceae) leaf MeOH extracts showed in vitro non‐cytotoxic and anti‐dengue virus serotype 2 (DENV2) activity in human hepatocarcinoma cell lineage (HepG2). Submitting these fractions to the developed RP‐SPE method allowed isolating the antiviral flavanone (2 S )‐isosakuranetin‐7‐ O ‐ β ‐ d ‐apiofuranosyl‐(1→6)‐ β ‐ d ‐glucopyranoside ( 1 ) from both species and yielded less active sub‐fractions. The new diastereoisomeric epimer pair (2 S ) + (2 R ) of 5,3′,5′‐trihydroxyflavanone‐7‐ O ‐ β ‐ d ‐apiofuranosyl‐(1→6)‐ β ‐ d ‐glucopyranoside ( 2a / 2b ) from F .  hyacinthina ; the known narigenin‐7‐ O ‐ β ‐ d ‐apiofuranosyl‐(1→6)‐ β ‐ d ‐glucopyranoside ( 3 ) from both species; rutin ( 4 ) and quercetin‐4′‐ β ‐ d ‐ O ‐glucopyranosyl‐3‐ O ‐rutinoside ( 5 ) from F .  hyacinthina , and kaempferol‐3‐ O ‐rutinoside ( 6 ), erythroxyloside A ( 7 ) and asperuloside ( 8 ) from F .  truncata have been isolated from these sub‐fractions. Compounds 4  –  8 are reported for the first time in Faramea spp.