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Discrimination and Characterization of Two Mediterranean Species from the Laurencia Complex (Rhodomelacea) Using an NMR ‐Based Metabolomic Approach
Author(s) -
Sutour Sylvain,
Esselin Hélène,
Bighelli Ange,
Casanova Joseph,
Le Gall Line,
Tomi Félix
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700226
Subject(s) - laurencia , metabolomics , chemistry , dna barcoding , computational biology , population , stereochemistry , biology , evolutionary biology , chromatography , botany , algae , demography , sociology
Generic and specific determination among the Laurencia complex is a challenging task. DNA barcoding combined with phenotypic investigations are mandatory for species differentiation. In this study, two morphologically different members of the Laurencia complex were investigated using untargeted 1 H‐ NMR ‐based metabolomics. Twenty‐one population samples were collected in order to evaluate both temporal and geographical homogeneity. Data obtained from 1 H‐ NMR analysis followed by statistical analysis allowed a clear separation of all the samples into two groups. DNA mitochondrial tests confirmed this pattern and identified the two species as Laurenciella sp. and Laurencia obtusa . In addition, metabolites responsible of this discrimination were investigated directly in crude extracts by 13 C‐ NMR using an in‐house computer‐assisted method. The combination of both untargeted ( 1 H) and targeted ( 13 C) NMR ‐based metabolomic approaches proves to be a powerful and complementary approach to discriminate species from the Laurencia complex.