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Exploration of Phytoconstituents from Mussaenda roxburghii and Studies of Their Antibiofilm Effect
Author(s) -
Ghosh Ranjit,
Das Manash C.,
Sarkar Arpita,
Das Antu,
Sandhu Padmani,
Dinda Biswanath,
Akhter Yusuf,
Bhattacharjee Surajit,
De Utpal Ch.
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700165
Subject(s) - chemistry , phytochemical , pseudomonas aeruginosa , antibacterial activity , context (archaeology) , minimum inhibitory concentration , biofilm , lupeol , swarming motility , traditional medicine , microbiology and biotechnology , quorum sensing , biochemistry , in vitro , bacteria , biology , medicine , paleontology , genetics , virulence , gene
In the context of ethno botanical importance with no phytochemical investigations, Mussaenda roxburghii have been investigated to explore it's phytoconstituents and studies of their antibiofilm activity. Four compounds have been isolated from the aerial parts of this plant and were characterized as 2 α ,3 β ,19 α ,23‐tetrahydroxyurs‐12‐en‐28‐oic acid ( 1 ), β ‐sitosterol glucoside ( 4 ), lupeol palmitate ( 5 ), and myoinositol ( 6 ). All these compounds were tested for antibacterial and antibiofilm activity against Pseudomonas aeruginosa . Compound 1 exhibited three times more antibiofilm activity with minimum inhibitory concentration ( MIC ) at 0.74 m m compared to that of streptomycin. Molecular docking studies exhibited a very high binding affinity of 1 with P. aeruginosa quorum sensing proteins and motility associated proteins viz . LasR and PilB, PilY1, PilT, respectively. Compound 1 was also found to be non‐cytotoxic against sheep RBC and murine peritoneal macrophages at selected sub‐ MIC doses.