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UHPLC / HR ‐ ESI ‐ MS / MS Profiling of Phenolics from Tunisian Lycium arabicum Boiss . Antioxidant and Anti‐lipase Activities’ Evaluation
Author(s) -
Affes Marwa,
Fakhfakh Jawhar,
Daoud Ines,
Brieudes Vincent,
Halabalaki Maria,
El Feki Abdelfattah,
Allouche Noureddine
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700095
Subject(s) - chemistry , dpph , phytochemical , lycium , antioxidant , rutin , flavonoid , lipase , food science , linoleic acid , polyphenol , fatty acid , biochemistry , enzyme , medicine , alternative medicine , pathology
This study was performed in the aim to evaluate nine different extracts from Tunisian Lycium arabicum for their total phenolic and total flavonoid contents, phytochemical analyses as well as their antioxidant and anti‐lipase activities. The in vitro antioxidant property was investigated using three complementary methods ( DPPH , ferric reducing antioxidant power ( FRAP ), and β ‐carotene‐linoleic acid bleaching assays) while anti‐lipase activity was evaluated using 4‐methylumbelliferyl oleate method. From all of the tested extracts the most potent found to be the polar Me OH extracts especially those of stems and leaves. In order to investigate the chemical composition of these extracts and possible correlation of their constituents with the observed activities, an UHPLC / HR ‐ ESI ‐ MS / MS analysis was performed. Several compounds belonging to different chemical classes were tentatively identified such as rutin and kampferol rutinoside, the major constituents of the leaves, and N ‐caffeoyltyramine, lyciumide A, N ‐dihydrocaffeoyltyramine as well as fatty acids: trihydroxyoctadecadienoic acid and hydroxyoctadecadienoic acid isomers were detected abundantly in the stems. These results showed that the Me OH extracts of stems and leaves of L . arabicum can be considered as a potential source of biological active compounds.