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Antioxidant Capacity, Phenolic Constituents and Toxicity of Hot Water Extract from Red Maple Buds
Author(s) -
Meda Naamwin R.,
Poubelle Patrice E.,
Stevanovic Tatjana
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201700028
Subject(s) - chemistry , dpph , antioxidant , quercetin , antioxidant capacity , toxicity , food science , high performance liquid chromatography , chromatography , biochemistry , organic chemistry
The present study reports, for the first time, the results of the antioxidant capacity and the phenolic composition of a hot water extract from red maple buds ( RMB ), as well as its safety. In this regard and comparatively to antioxidant standards, this extract exhibits a significant antiradical capacity when tested by 2,2‐diphenyl‐1‐picrylhydrazyl ( DPPH · ) and anion superoxide trapping assays. High‐resolution mass spectrometric and nuclear magnetic resonance analyses permitted to determine for the first time, in red maple species, cyanidin‐3‐ O ‐glucoside, quercetin‐3‐ O ‐galactoside, quercetin‐3‐ O ‐arabinoside, and quercetin. Also, the quantification of individual phenolics by high‐performance liquid chromatography method revealed that ginnalin A at 117.0 mg/g is the major compound of RMB hot water extract. Finally, using flow cytometry evaluation, the extract of RMB was determined to have no toxicity neither to cause significant modification of apoptosis process, up to concentration of 100 μg/ml, on human peripheral blood neutrophils. These results allow anticipating various fields of application of RMB water extract.