Premium
Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves ( Morus alba L.) before and after Frost
Author(s) -
Yu Xiaofeng,
Zhu Yiling,
Fan Jingyi,
Wang Dujun,
Gong Xiaohui,
Ouyang Zhen
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600496
Subject(s) - flavonoid , chemistry , astragalin , gene expression , glycoside , botany , gene , biochemistry , stereochemistry , biology , kaempferol , antioxidant
In order to determine the molecular mechanism underlying the influence of frost on chemical changes in mulberry leaves, the UFGT activity, expression level, and accumulation of flavonoid glycosides in mulberry leaves ( Morus alba L.) were studied. The expression of UFGT gene was investigated by quantitative real‐time PCR ( qRT ‐ PCR ) and the UFGT activity, accumulation of flavonoid glycosides was studied by high performance liquid chromatography. Then, the correlation between the expression level of UFGT , the UFGT activity, and the flavonoid glycosides accumulation with temperature was explored. The accumulation of isoquercitrin and astragalin is significantly positively correlated with UFGT gene expression and UFGT activity. On the contrary, the average temperature was significantly negatively correlated with the level of UFGT gene expression and UFGT activity. The results show that after frost, low temperature can induce the expression of UFGT gene in mulberry leaves, resulting in the accumulation of flavonoid glycosides.