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Flavonoids from Potentilla parvifolia Fisch . and Their Neuroprotective Effects in Human Neuroblastoma SH ‐ SY 5Y Cells in vitro
Author(s) -
Yuan Zhenzhen,
Luan Guangxiang,
Wang Zhenhua,
Hao Xueyan,
Li Ji,
Suo Yourui,
Li Gang,
Wang Honglun
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600487
Subject(s) - neuroprotection , chemistry , potentilla , in vitro , astragalin , biochemistry , pharmacology , flavonoid , kaempferol , botany , biology , antioxidant
Potentilla parvifolia Fisch . (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin ( 1 ), tricin ( 2 ), quercetin ( 3 ), tiliroside ( 4 ), miquelianin ( 5 ), isoquercitrin ( 6 ), and astragalin ( 7 ), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH ‐ SY 5Y cells were differentiated with all trans‐retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects.