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Inhibitory Activities of Butanol Fraction from Butea monosperma ( Lam .) Taub . Bark Against Free Radicals, Genotoxins and Cancer Cells
Author(s) -
Kaur Varinder,
Kumar Manish,
Kaur Paramjeet,
Kaur Sandeep,
Kaur Satwinderjeet
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600484
Subject(s) - chemistry , apoptosis , comet assay , lipid peroxidation , gallic acid , genotoxicity , antioxidant , biochemistry , abts , cancer cell , dna damage , flow cytometry , ellagic acid , polyphenol , pharmacology , traditional medicine , microbiology and biotechnology , dpph , biology , cancer , toxicity , dna , medicine , genetics , organic chemistry
The present study was undertaken to investigate antioxidant, antigenotoxic, and antiproliferative activity of butanol fraction ( Bmbu ) from bark of medicinal plant Butea monosperma . Antioxidant potency of Bmbu was examined by various in vitro assays. It was also investigated for antigenotoxic activity using Escherichia coli . PQ 37 employing SOS chromotest. Further, cytotoxic and apoptosis inducing activity of Bmbu was evaluated in MCF ‐7 breast cancer cells. Bmbu showed potent free radical scavenging ability in ABTS assay ( IC 50 56.70 μg/ml) and anti‐lipid peroxidation ability ( IC 50 40.39 μg/ml). 4 NQO and H 2 O 2 induced genotoxicity was suppressed by Bmbu in SOS chromotest by 74.26% and 82.02% respectively. It also inhibited the growth of MCF ‐7 cells with GI 50 value of 158.71 μg/ml. Induction of apoptosis in MCF ‐7 cells by Bmbu treatment was deciphered using confocal microscopy, flow cytometry, and neutral comet assay. Bmbu treatment increased cell population in sub‐G 1 phase (69.6%) indicating apoptotic cells. Further, Bmbu treatment resulted in increased reactive oxygen species generation and decreased mitochondrial membrane potential indicating involvement of mitochondrial dependent pathway of apoptosis. HPLC profiling showed the presence of polyphenols such as ellagic acid, catechin, quercetin, and gallic acid as its major constituents. Consequently, it is suggested that the phytoconstituents from this plant may be further exploited for development of novel drug formulation with possible therapeutic implication.