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Laserpitium zernyi Hayek Flower and Herb Extracts: Phenolic Compounds, and Anti‐edematous, Antioxidant, and Antimicrobial Activities
Author(s) -
Popović Višnja B.,
Tomić Maja A.,
StepanovićPetrović Radica M.,
Micov Ana M.,
Milenković Marina T.,
Petrović Silvana D.,
Ušjak Ljuboš J.,
Niketić Marjan S.
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600432
Subject(s) - micrococcus luteus , chemistry , antimicrobial , dpph , apigenin , herb , polyphenol , luteolin , antioxidant , traditional medicine , broth microdilution , antibacterial activity , enterococcus faecalis , candida albicans , food science , flavonoid , bacteria , microbiology and biotechnology , minimum inhibitory concentration , biochemistry , biology , medicinal herbs , organic chemistry , escherichia coli , medicine , genetics , gene
Phenolic compounds and different biological activities of the dry Me OH extracts of the flowers and the herb (aerial parts without flowers) of Laserpitium zernyi Hayek (Apiaceae) were investigated. The total phenolic contents in the extracts were determined spectrophotometrically using Folin–Ciocalteu reagent. In both extracts, apigenin, luteolin, their 7‐ O ‐glucosides, and chlorogenic acid were detected by HPLC . Identified phenolics were quantified in both extracts, except luteolin in L . zernyi herb extract. The extracts ( p.o .) were tested for anti‐edematous activity in a model of carrageenan ( i.pl .) induced rat paw edema. Antioxidant activity of the extracts was assessed by FRAP assay and DPPH and • OH radicals scavenging tests. Antimicrobial activity was investigated using broth microdilution test against five Gram ‐positive and three Gram ‐negative bacteria, as well as against two strains of Candida albicans . The polyphenol‐richer flower extract exerted higher anti‐edematous and antioxidant activities. The herb extract exhibited better antimicrobial effect against Micrococcus luteus , Enterococcus faecalis , Bacillus subtilis , and Pseudomonas aeruginosa , while against other tested microorganisms, the activity of both extracts was identical. Demonstrated biological activities of L . zernyi flower and herb extracts represent a good basis for their further investigation as potential new herbal medicinal raw materials.