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Chemical Composition, Antimicrobial Activity, and Mode of Action of Essential Oils against Paenibacillus larvae , Etiological Agent of American Foulbrood on Apis mellifera
Author(s) -
Pellegrini María C.,
AlonsoSalces Rosa M.,
Umpierrez María L.,
Rossini Carmen,
Fuselli Sandra R.
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600382
Subject(s) - american foulbrood , essential oil , antimicrobial , carvone , limonene , chemistry , botany , food science , pyrilamine , biology , biochemistry , organic chemistry , larva , receptor , antagonist
This study aimed to characterize the chemical composition of Aloysia polystachia , Acantholippia seriphioides , Schinus molle , Solidago chilensis , Lippia turbinata , Minthostachys mollis , Buddleja globosa , and Baccharis latifolia essential oils ( EO s), and to evaluate their antibacterial activities and their capacity to provoke membrane disruption in Paenibacillus larvae , the bacteria that causes the American Foulbrood ( AFB ) disease on honey bee larvae. The relationship between the composition of the EO s and these activities on P . larvae was also analyzed. Monoterpenes were the most abundant compounds in all EO s. All EO s showed antimicrobial activity against P . larvae and disrupted the cell wall and cytoplasmic membrane of P . larvae provoking the leakage of cytoplasmic constituents (with the exception of B . latifolia EO ). While, the EO s’ antimicrobial activity was correlated most strongly to the content of pulegone, carvone, ( Z )‐ β ‐ocimene, δ ‐cadinene, camphene, terpinen‐4‐ol, elemol, β ‐pinene, β ‐elemene, γ ‐cadinene, α ‐terpineol, and bornyl acetate; the volatiles that better explained the membrane disruption were carvone, limonene, cis ‐carvone oxide, pentadecane, trans ‐carvyl acetate, trans ‐carvone oxide, trans ‐limonene oxide, artemisia ketone, trans ‐carveol, thymol, and γ ‐terpinene (positively correlated) and biciclogermacrene, δ ‐2‐carene, verbenol, α ‐pinene, and α ‐thujene (negatively correlated). The studied EO s are proposed as natural alternative means of control for the AFB disease.