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In vitro Activities of Pfaffia glomerata Root Extract, Its Hydrolyzed Fractions and Pfaffic Acid Against Trypanosoma cruzi Trypomastigotes
Author(s) -
Silva Márcio L. A. e,
Pereira Ana C.,
Ferreira Daniele S.,
Esperandim Viviane R.,
Símaro Guilherme V.,
Lima Thaís C.,
Januário Ana H.,
Pauletti Patrícia M.,
Rehder Vera L. G.,
Crevelin Eduardo J.,
Cunha Wilson R.,
Crotti Antônio E. M.,
Bastos Jairo K.
Publication year - 2017
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600175
Subject(s) - trypanosoma cruzi , in vitro , chemistry , hydrolysis , biochemistry , traditional medicine , botany , microbiology and biotechnology , parasite hosting , biology , medicine , world wide web , computer science
This article reports on the in vitro activity of the hydroalcoholic extract of Pfaffia glomerata roots, its hydrolyzed fractions, and pfaffic acid against Trypanosoma cruzi . The hydroalcoholic extract obtained from dried, milled P . glomerata roots was submitted to acid hydrolysis followed by partition with CHC l 3 . The concentrated CHC l 3 fraction was suspended in Me OH /H 2 O and partitioned with hexane ( F1 ), CHC l 3 ( F2 ), and Ac OE t ( F3 ), in this sequence. The trypanocidal activity of the hydrolyzed extract and its fractions was evaluated in vitro . The hydroalcoholic extract displayed low activity, but fraction F1 was active against trypomastigotes of the Y strain of T . cruzi , with IC 50 = 47.89 μg/ml. The steroids campesterol (7.7%), stigmasterol (18.7%), β ‐sitosterol (16.8%), Δ 7 ‐stigmastenol (4.6%), and Δ 7 ‐spinasterol (7.5%) were the major constituents of F1 , along with fatty acid esters (7.6%) and eight aliphatic hydrocarbons (30.1%). Fractions F2 and F3 exhibited moderate activity, and pfaffic acid, one of the main chemical constituents of these fractions, displayed IC 50 = 44.78 μ m (21.06 μg/ml). On the other hand, the hydroalcoholic extract of P . glomerata roots, which is rich in pfaffosides, was inactive. Therefore, the main aglycone of pfaffosides, pfaffic acid, is much more active against trypomastigotes of the Y strain of T . cruzi than its corresponding glycosides and should be further investigated.