Premium
Chemical Profiling of the Essential Oils of Syzygium aqueum , Syzygium samarangense and Eugenia uniflora and Their Discrimination Using Chemometric Analysis
Author(s) -
Sobeh Mansour,
Braun Markus Santhosh,
Krstin Sonja,
Youssef Fadia S.,
Ashour Mohamed L.,
Wink Michael
Publication year - 2016
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600089
Subject(s) - syzygium , essential oil , myrtaceae , antimicrobial , chemistry , botany , trypanosoma brucei , traditional medicine , biology , food science , biochemistry , organic chemistry , medicine , gene
The essential oil compositions of the leaves of three related Myrtaceae species, namely Syzygium aqueum , Syzygium samarangense and Eugenia uniflora , were investigated using GLC / MS and GLC / FID . Altogether, 125 compounds were identified: α ‐Selinene (13.85%), β ‐caryophyllene (12.72%) and β ‐selinene constitute the most abundant constituents in S . aqueum . Germacrene D (21.62%) represents the major compound in S . samarangense whereas in E . uniflora , spathulenol (15.80%) represents the predominant component. Multivariate chemometric analyses were used to discriminate the essential oils using hierarchical cluster analysis ( HCA ) and principal component analysis ( PCA ) based on the chromatographic results. The antimicrobial activity of the popularly used E . uniflora essential oil was assessed using broth microdilution method against six Gram ‐positive, three Gram ‐negative bacteria and two fungi. The oil showed moderate antimicrobial activity against Bacillus licheniformis exhibiting MIC and MMC of 0.63 mg/ml. The cytotoxic activity of E . uniflora essential oil was investigated against Trypanosoma brucei brucei ( T . b . brucei ) and MCF ‐7 cancer cell line using MTT assay. It showed moderate activity against MCF ‐7 cells with an IC 50 value of 76.40 μg/ml. On the other hand, T . brucei was highly susceptible to E . uniflora essential oil with IC 50 of 11.20 μg/ml, and a selectivity index of 6.82.