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Secondary Metabolites, Glandular Trichomes and Biological Activity of Sideritis montana L. subsp. montana from Central Italy
Author(s) -
Venditti Alessandro,
Bianco Armandodoriano,
Frezza Claudio,
Serafini Mauro,
Giacomello Ginevra,
Giuliani Claudia,
Bramucci Massimo,
Quassinti Luana,
Lupidi Giulio,
Lucarini Domenico,
Papa Fabrizio,
Maggi Filippo
Publication year - 2016
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600082
Subject(s) - trichome , chemistry , essential oil , botany , dpph , indumentum , abts , terpene , asteraceae , genus , lamiaceae , antioxidant , stereochemistry , chromatography , biology , biochemistry
Sideritis montana subsp. montana is a small annual herb occurring in countries bordering the Mediterranean and Balkan regions. The secondary metabolism of this plant has not been fully explored so far. The aim of the present study was to understand the complex mixture of secondary metabolites and the type of secretory structures. The polar constituents were isolated by column chromatography from the ethanolic extract, and their structure was elucidated by NMR and MS . The essential oil was isolated by hydrodistillation and analysed by GC / MS . The plant indumentum was studied by light and scanning electron microscopy. To complete the work, the essential oil antioxidant activity and cytotoxicity on tumor cells were evaluated by DPPH , ABTS , FRAP , and MTT methods. Four different classes of secondary metabolites were isolated, namely flavonoids, caffeoylquinic derivatives, glycosidic hydroquinones and iridoids. The essential oil was mainly characterized by sesquiterpenene hydrocarbons. Peltate and long‐capitate hairs were the main sites where terpenes and polar constituents are produced. The secondary metabolites found in S. montana subsp. montana are of chemotaxonomic interest, some of them being typical of the genus Sideritis . The trichomes types observed partially differ from those described in other members of the genus Sideritis . The essential oil showed noteworthy inhibition on tumor cells.