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Antimicrobial Activity of Scabiosa arenaria Forssk . Extracts and Pure Compounds Using Bioguided Fractionation
Author(s) -
Besbes Hlila Malek,
Mosbah Habib,
Majouli Kaouther,
Ben Nejma Aymen,
Ben Jannet Hichem,
Mastouri Maha,
Aouni Mahjoub,
Selmi Boulbaba
Publication year - 2016
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201600028
Subject(s) - chemistry , fractionation , antimicrobial , botany , chromatography , biology , organic chemistry
The emergence of multidrug resistant pathogens threatened the clinical efficacy of many existing antibiotics. This situation has been recognized globally as a serious concern and justifies further research to discover antimicrobial agents from natural origins including plant extracts. The aim of our work was to evaluate the antimicrobial activities of Scabiosa arenaria Forssk . extracts and pure compounds using a bioguided fractionation, and try to explain some traditional use of this genus. The best antimicrobial activity‐guided fractionation was obtained by Bu OH fractions of flowers, fruits and (stems and leaves) against Escherichia coli , Pseudomonas aeruginosa and Candida albicans with minimum inhibitory concentration ( MIC ) values from 0.0195 to 5 mg/ml. Escherichia coli was the most affected bug, thus the MIC of fruits Bu OH extract showed the best anti‐ Escherichia coli activity ( MIC = 0.0195 mg/ml), followed by the (stems and leaves) and flowers Bu OH extracts; MIC = 0.078 and 0.15 mg/ml, respectively. Furthermore, the subfractions obtained from these three mixed fractions showed also an important antimicrobial activity against the three microorganisms, with MIC values between 0.0195 and 0.312 mg/ml. The fractionation of the aerial part Bu OH fraction led to the isolation of oleanolic acid ( 1 ) and luteolin 7‐ O ‐glucopyranoside ( 2 ) which are reported here for the first time from S . arenaria . Both compounds showed good antimicrobial activities with MIC values ranging from 170 to 683 μ m and 86 to 347 μ m , respectively. These results support the use of the Scabiosa genus to inhibit the growth of tested pathogenic bacteria and yeasts which may reduce illnesses associated with their exposure.