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Structure Elucidation, Antimicrobial and Cytotoxic Activities of a Halimane Isolated from Vellozia kolbekii Alves (Velloziaceae)
Author(s) -
Silva Carmelita G.,
Santos Júnior Helvécio M.,
Barbosa Jussara P.,
Costa Gisela L.,
Rodrigues Felipe A. R.,
Oliveira Denilson F.,
CostaLotufo Letícia V.,
Alves Ruy J. V.,
Eleutherio Elis C. A.,
Rezende Claudia M.
Publication year - 2015
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201500071
Subject(s) - bacillus cereus , cytotoxicity , antimicrobial , chemistry , escherichia coli , cytotoxic t cell , bacillus subtilis , microbiology and biotechnology , chloramphenicol , apoptosis , mitosis , bacteria , biochemistry , biology , antibiotics , in vitro , gene , genetics
A new halimane diterpene was isolated from Vellozia kolbekii Alves (Velloziaceae) and identified as (5 R ,8 R ,9 S ,13 R )‐halim‐1,10‐ene‐15,16‐diol ( 1 ). It showed cytotoxicity against three human cancer cell lines, SF‐295 (glioblastoma), MDA‐MB‐435 (melanoma), and HCT‐8 (colon adenocarcinoma). In the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in S phase, in which DNA synthesis occurs and where it is very sensitive to damage, and G2M phase which is the phase of preparation for mitosis and mitosis itself, showing apoptosis‐inducing properties. Antimicrobial activity towards Gram ‐positive and Gram ‐negative bacteria was studied and, against Bacillus cereus, B. subtilis, Escherichia coli , and Pseudomonas aeruginosa , a MIC value of 0.025 μ M was observed for halimane 1 , which is more active than the positive control chloramphenicol.