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Application of NMR Spectroscopy in the Assessment of Radiation Dose in Human Primary Cells
Author(s) -
Kang ChangMo,
Seong Hyeon Jin,
Ra Kim So,
Kyeong Lee Eun,
Jin Yun Hyun,
Young Kim Sun,
Kee Chae Young
Publication year - 2015
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201400431
Subject(s) - alanine , chemistry , ionizing radiation , irradiation , metabolomics , glycine , nuclear magnetic resonance spectroscopy , metabolite , primary metabolite , spectroscopy , radiochemistry , amino acid , biochemistry , stereochemistry , chromatography , physics , quantum mechanics , nuclear physics
We employed the primary cell model system as a first step toward establishing a method to assess the influence of ionizing radiation by using a combination of common and abundant metabolites. We applied X‐ray irradiation amounts of 0, 1, and 5 Gy to the cells that were harvested 24, 48, or 72 h later, and profiled metabolites by 2D‐NMR spectroscopy to sort out candidate molecules that could be used to distinguish the samples under different irradiation conditions. We traced metabolites stemming from the input 13 C‐glucose, identified twelve of them from the cell extracts, and applied statistical analysis to find out that all the metabolites, including glycine, alanine, and gluatamic acid, increased upon irradiation. The combinatorial use of the selected metabolites showed promising results where the product of signal intensities of alanine and lactate could differentiate samples according to the dose of X‐ray irradiation. We hope that this work can form a base for treating radiation‐poisoned patients in the future.