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Free‐Radical‐Scavenging, Antityrosinase, and Cellular Melanogenesis Inhibitory Activities of Synthetic Isoflavones
Author(s) -
Lu TzyMing,
Ko HorngHuey,
Ng LeanTeik,
Hsieh YenPin
Publication year - 2015
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201400208
Subject(s) - tyrosinase , chemistry , isoflavones , hacat , cosmeceutical , dpph , melanin , biochemistry , ic50 , mushroom , garcinia mangostana , free radical scavenger , pharmacology , fucoidan , traditional medicine , in vitro , antioxidant , food science , enzyme , biology , polysaccharide , medicine , microbiology and biotechnology
In this study, we examined the potential of synthetic isoflavones for application in cosmeceuticals. Twenty‐five isoflavones were synthesized and their capacities of free‐radical‐scavenging and mushroom tyrosinase inhibition, as well as their impact on cell viability of B16F10 murine melanoma cells and HaCaT human keratinocytes were evaluated. Isoflavones that showed significant mushroom tyrosinase inhibitory activities were further studied on reduction of cellular melanin formation and antityrosinase activities in B16F10 melanocytes in vitro. Among the isoflavones tested, 6‐hydroxydaidzein ( 2 ) was the strongest scavenger of both ABTS . + and DPPH . radicals with SC 50 values of 11.3±0.3 and 9.4±0.1 μ M , respectively. Texasin ( 20 ) exhibited the most potent inhibition of mushroom tyrosinase ( IC 50 14.9±4.5 μ M ), whereas retusin ( 17 ) showed the most efficient inhibition both of cellular melanin formation and antityrosinase activity in B16F10 melanocytes, respectively. In summary, both retusin ( 17 ) and texasin ( 20 ) exhibited potent free‐radical‐scavenging capacities as well as efficient inhibition of cellular melanogenesis, suggesting that they are valuable hit compounds with potential for advanced cosmeceutical development.