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Bioprospection of Cytotoxic Compounds in Fungal Strains Recovered from Sediments of the Brazilian Coast
Author(s) -
Rodrigues Bárbara S. F.,
Sahm Bianca D. B.,
Jimenez Paula C.,
Pinto Francisco C. L.,
Mafezoli Jair,
Mattos Marcos C.,
RodriguesFilho Edson,
Pfenning Ludwig H.,
Abreu Lucas M.,
CostaLotufo Letícia V.,
Oliveira Maria C. F.
Publication year - 2015
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201400193
Subject(s) - gliotoxin , cytotoxic t cell , chemistry , fermentation , bioassay , cytotoxicity , strain (injury) , chromatography , stereochemistry , biochemistry , microbiology and biotechnology , biology , in vitro , aspergillus fumigatus , genetics , anatomy
The cytotoxic activities of extracts (50 μg/ml) from 48 fungal strains, recovered from sediments of Pecém's offshore port terminal (Northeast coast of Brazil), against HCT‐116 colon cancer cell lines were investigated. The most promising extract was obtained from strain BRF082, identified as Dichotomomyces cejpii by phylogenetic analyses of partial RPB2 gene sequence. Thus, it was selected for bioassay‐guided isolation of the cytotoxic compounds. Large‐scale fermentation of BRF082 in potato dextrose broth, followed by chromatographic purification of the bioactive fractions from the liquid medium, yielded gliotoxin ( 4 ) and its derivatives acetylgliotoxin G ( 3 ), bis(dethio)bis(methylsulfanyl)gliotoxin ( 1 ), acetylgliotoxin ( 5 ), 6‐acetylbis(dethio)bis(methylsulfanyl)gliotoxin ( 2 ), besides the quinazolinone alkaloid fiscalin B. All isolated compounds were tested for their cytotoxicities against the tumor cell lines HCT‐116, revealing 4 and 3 as the most cytotoxic ones ( IC 50 0.41 and 1.06 μg/ml, resp.).