Premium
Suppression of IL‐2 Production and Proliferation of CD4 + T Cells by Tuberostemonine O
Author(s) -
Jung Jang Eun,
Kil YunSeo,
Ryeon Park Hye,
Oh Sera,
Kyeong Kim Hyo,
Gyeong Jeong Mi,
Kyoung Seo Eun,
Sook Hwang Eun
Publication year - 2014
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201400074
Subject(s) - chemistry , nitric oxide , cytokine , alkaloid , recombinant dna , inflammation , cell growth , t cell , stereochemistry , microbiology and biotechnology , biochemistry , immunology , immune system , biology , organic chemistry , gene
Tuberostemonine stereoisomers are natural alkaloids found in Stemona tuberosa , that are known to have anti‐inflammatory and anti‐infective properties. Tuberostemonine alkaloids inhibit inflammation by suppressing the expression of inflammatory mediators such as cyclooxygenase and nitric oxide synthase. However, the direct immunomodulatory properties of tuberostemonine alkaloids in T cells have not been elucidated so far. In this study, the activities in T cells of tuberostemonine N (TbN) and a novel alkaloid, tuberostemonine O (TbO), isolated from S. tuberosa , were investigated. Although TbN did not have a significant effect on cytokine production in splenic T cells, TbO selectively suppressed interleukin (IL)‐2 production. Moreover, TbO, but not TbN, significantly inhibited IL‐2 production by primary CD4 + T cells and delayed the T‐cell proliferation in a dose‐dependent manner. Addition of excess recombinant IL‐2 restored the decreased cell‐division rates in TbO‐treated CD4 + T cells to control levels. Collectively, these findings suggest that the immunomodulatory effects of TbO occurred by the suppression of IL‐2 expression and IL‐2‐induced T‐cell proliferation, suggesting a potential beneficial role of tuberostemonine alkaloids for the control of chronic inflammatory and autoimmune diseases caused by hyperactivated T cells.