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Anti‐Adipogenic Activity of the Naturally Occurring Phenanthroindolizidine Alkaloid Antofine via Direct Suppression of PPAR γ Expression
Author(s) -
Jang Eun Jung,
Kim Hyo Kyeong,
Jeong Hana,
Lee Ye Seul,
Jeong Mi Gyeong,
Bae Su Jung,
Kim Sanghee,
Lee Sang Kook,
Hwang Eun Sook
Publication year - 2014
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201300365
Subject(s) - adipogenesis , adipocyte , downregulation and upregulation , chemistry , alkaloid , transcription factor , gene expression , peroxisome proliferator activated receptor , microbiology and biotechnology , peroxisome , inflammation , gene , pharmacology , biochemistry , medicine , adipose tissue , biology , stereochemistry
Antofine (ANTF) is a phenanthroindolizidine alkaloid isolated from the root of Cynanchum paniculatum Kitagawa (Asclepiadaceae), which is used as an herbal remedy for pain and inflammation. ANTF also possesses antiviral and antitumorigenic activities. In this study, we investigated the role of ANTF in adipogenesis. Chronic ABTF administration suppressed adipocyte differentiation and marker expression in a dose‐dependent manner. Furthermore, acute administration of ANTF at early stages of differentiation process inhibited lipid droplet formation and adipogenic gene expression. ANTF Treatment decreased expression of PPAR γ protein, a master transcription factor in the regulation of adipocyte differentiation, leading to a suppression of aP2 promoter activity. These results suggest that ANTF exerts potent anti‐adipogenic effects via direct suppression of PPAR γ protein expression, with consequent downregulation of adipogenic gene expression.