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Composition and Activity against Oral Pathogens of the Essential Oil of Melampodium divaricatum ( Rich .) DC.
Author(s) -
Duarte Moreira Raquel Regina,
Zimmermann Martins Gilmarcio,
Teixeira Botelho Vinícius,
dos Santos Luis Eduardo,
Cavaleiro Carlos,
Salgueiro Lígia,
Andrade Géssica,
Martins Carlos Henrique Gomes
Publication year - 2014
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201300322
Subject(s) - streptococcus sobrinus , germacrene d , essential oil , antimicrobial , chemistry , streptococcus mutans , food science , broth microdilution , lactobacillus casei , minimum inhibitory concentration , traditional medicine , microbiology and biotechnology , biology , bacteria , medicine , genetics , fermentation , organic chemistry
The chemical composition of the essential oil isolated from the aerial parts of Melampodium divaricatum ( Rich .) DC. (Asteraceae) was characterized by GC‐FID and GC/MS analyses. ( E )‐Caryophyllene (56.0%), germacrene D (12.7%), and bicyclogermacrene (9.2%) were identified as the major oil components. The antimicrobial activity of the oil against seven standard strains of oral pathogens from the American Type Culture Collection (ATCC) was evaluated by determining minimum inhibitory concentrations ( MIC s) using the microdilution method. MIC Values below 100 μg/ml were obtained against Streptococcus sobrinus (90 μg/ml), Lactobacillus casei (30 μg/ml), S. mutans (20 μg/ml), and S. mitis (18 μg/ml). In contrast, the MIC values of the major oil compound ( E )‐caryophyllene were higher than 400 μg/ml against all pathogens, suggesting that the activity of the oil might depend on minor oil components and/or on synergistic effects. The M. divaricatum essential oil is a promising agent to include in anticariogenic oral rinse formulations for the control of oral pathogens.