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Chemical Composition and Antimicrobial Activity of the Essential Oil of Stachys officinalis (L.) Trevis . (Lamiaceae)
Author(s) -
Lazarević Jelena S.,
Ðorđević Aleksandra S.,
Kitić Dušanka V.,
Zlatković Bojan K.,
Stojanović Gordana S.
Publication year - 2013
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201200332
Subject(s) - lamiaceae , antimicrobial , chemistry , composition (language) , officinalis , traditional medicine , essential oil , chemical composition , botany , food science , biology , organic chemistry , medicine , linguistics , philosophy
Characterization by GC‐FID and GC/MS analyses of the Stachys officinalis (L.) Trevis . essential oil obtained by hydrodistillation of the aerial parts allowed the identification of 190 components that represented 97.9% of the total oil content. The main constituents identified were germacrene D (19.9%), β ‐caryophyllene (14.1%), and α ‐humulene (7.5%). Terpenoids were by far predominant (89.4%), with sesquiterpene hydrocarbons (69.1%) and oxygenated sesquiterpenes (14.8%) being the most abundant compounds detected in the oil. Based on the present and previously published results, multivariate statistical comparison of the chemical composition of the essential oils was performed within the species. Principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) of the data on the volatile profiles of S. officinalis taxa revealed no pronounced differences among the samples originated from the Balkan Peninsula. Additionally, the oil was screened for in vitro antibacterial and antifungal activity using the broth microdilution assay. The oil's best antimicrobial activities were obtained against the mold Aspergillus niger (minimal inhibitory ( MIC ) and minimal fungicidal ( MFC ) concentrations of 2.5 and 5.0 mg/ml, resp.) and the yeast Candida albicans ( MIC and MFC of 5.0 mg/ml).