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Chemical Constituents from a Soil‐Derived Actinomycete, Actinomadura miaoliensis BCRC 16873, and Their Inhibitory Activities on Lipopolysaccharide‐Induced Tumor Necrosis Factor Production
Author(s) -
Tseng Min,
Su YungShun,
Cheng MingJen,
Liu TaWei,
Chen IhSheng,
Wu MingDer,
Chang HsunShuo,
Yuan GwoFang
Publication year - 2013
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201200242
Subject(s) - chemistry , lipopolysaccharide , tumor necrosis factor alpha , fermentation , pyrone , microbiology and biotechnology , inhibitory postsynaptic potential , in vitro , biochemistry , pharmacology , stereochemistry , biology , immunology , neuroscience
An investigation on the secondary metabolites from the BuOH extract of the fermentation broth of the thermotolerant polyester‐degrading actinomycete Actinomadura miaoliensis BCRC 16873 was carried out. One previously undescribed α ‐pyrone (=pyran‐2‐one) derivative, designated as miaolienone ( 1 ), and a new butanolide, miaolinolide ( 2 ), together with 13 known compounds, 3 – 15 , were obtained. Their structures were established on the basis of extensive 1D‐ and 2D‐NMR analyses in combination with HR‐MS experiments. In addition, the isolated compounds 1 – 15 were evaluated for the inhibitory effects of the isolates on the production of tumor necrosis factor (TNF‐ α ) induced by lipopolysaccharide (LPS). Among the isolates, 1 and 2 significantly inhibited TNF‐ α production in U937 cells in vitro , and the IC 50 values were 0.59 and 0.76 μ M , respectively. Compounds 3 – 5 displayed moderate inhibitory activities on LPS‐induced TNF‐ α production.