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Simultaneous RP‐HPLC‐DAD Separation, and Determination of Flavonoids and Phenolic Acids in Plantago L. Species
Author(s) -
Jurišić Grubešić Renata,
Srečnik Goran,
Kremer Dario,
Vuković Rodríguez Jadranka,
Nikolić Toni,
VladimirKnežević Sanda
Publication year - 2013
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201200210
Subject(s) - hyperoside , chlorogenic acid , plantago , botany , plantaginaceae , chemistry , quercitrin , quercetin , caffeic acid , chromatography , rutin , chemotaxonomy , biology , biochemistry , taxonomy (biology) , antioxidant
A rapid reversed‐phase (RP) high‐performance liquid chromatography method was developed and applied for simultaneous separation, and determination of flavonoids and phenolic acids in eight Plantago L. taxa ( P. altissima L., P. argentea Chaix , P. coronopus L., P. holosteum Scop . ssp. depauperata Pilger , P. holosteum ssp. holosteum, P. holosteum ssp. scopulorum (Degen) Horvatić , P. lagopus L., and P. maritima L.) growing in Croatia. Chromatographic separation was carried out on Zorbax Eclipse XDB‐C18 using gradient elution with a H 2 O (pH 2.5, adjusted with CF 3 COOH) and MeCN mixture at 30°. The contents of analyzed phenolic compounds (% of the dry weight of the leaves, dw) varied among examined species: rutin (max. 0.024%, P. argentea ), hyperoside (max. 0.020%, P. lagopus ), quercitrin (max. 0.013%, P. holosteum ssp. holosteum ), quercetin (max. 0.028%, P. holosteum ssp. scopulorum ), chlorogenic acid (max. 0.115%, P. lagopus ), and caffeic acid (max. 0.046%, P. coronopus ). Isoquercitrin was detected only in P. argentea (0.020%), while isochlorogenic acid content was below limit of quantification in all investigated species. Multivariate analyses (UPGMA and PCA) showed significant differences in contents of investigated polyphenolic compounds between different Plantago taxa. Accordingly, investigated substances might be employed as chemotaxonomic markers in the study of the complex genus Plantago.