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Seasonal Variation on the Contents of Coumarin and Kaurane‐Type Diterpenes in Mikania laevigata and M. glomerata Leaves under Different Shade Levels
Author(s) -
Bertolucci Suzan Kelly V.,
Pereira Ana Bárbara D.,
Pinto José Eduardo B. P.,
Oliveira Alaíde B.,
Braga Fernão C.
Publication year - 2013
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201200166
Subject(s) - chemistry , coumarin , botany , biology , organic chemistry
Coumarin ( 1 ) and kaurane‐type diterpenes are considered the bioactive constituents of Mikania glomerata and M. laevigata , used in Brazil to treat respiratory affective disorders. The seasonal variation of 1 , ortho ‐coumaric acid ( 2 ), benzoylgrandifloric acid ( 3 ), cinnamoylgrandifloric acid ( 4 ), and kaurenoic acid ( 5 ) in leaves of both species, cultivated in full sunlight and under shade levels of 40 and 80%, was quantified by HPLC. Compound 2 was detected solely in M. laevigata in concentrations below the limit of quantification. Coumarin was not found in M. glomerata , whereas its concentration reached 0.94±0.24% ( w / w ) in M. laevigata farmed in summer under 80% shading. Both Mikania species produced higher amounts of kaurane diterpenes when cultivated in plenty of sunlight. Hence, maximum contents of 1 are reached in M. laevigata cultivated under high shading, but with reduced concentrations of 3 – 5 . Conversely, M. glomerata should be cultivated under full sunlight and harvested in winter for highest concentrations of kaurane‐type diterpenes.