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Study of SOD Mimic and Nucleic Acid Interaction Activity Exerted by Enrofloxacin‐Based Copper(II) Complexes
Author(s) -
Patel Mohan N.,
Bhatt Bhupesh S.,
Dosi Promise A.
Publication year - 2012
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201100372
Subject(s) - chemistry , agarose gel electrophoresis , copper , nuclear chemistry , superoxide dismutase , titration , dna , gel electrophoresis , puc19 , antibacterial activity , stereochemistry , inorganic chemistry , biochemistry , bacteria , enzyme , organic chemistry , plasmid , biology , genetics
Five new copper(II) complexes of type [Cu(erx)( L )Cl] (erx, enrofloxacin; thiophene‐2‐carbaldehyde ( L 1 ); pyridine‐2‐carbaldehyde ( L 2 ); 2,2′‐dipyridylamine ( L 3 ); 4,5‐diazafluoren‐9‐one ( L 4 ); bis(3,5‐dimethyl‐1‐pyrazolyl)methane ( L 5 )) have been synthesized and characterized by elemental analysis, reflectance, IR, and FAB‐MS. Complexes have been investigated for their interaction with calf thymus (CT) DNA utilizing the absorption‐titration method, viscometric and DNA thermal denaturation studies. The cleavage reaction on pUC19 DNA has been monitored by agarose gel electrophoresis. The results indicated that the Cu II complexes can more effectively promote the cleavage of plasmid DNA at physiological pH and superoxide dismutase. The (SOD) activity of the complexes has been evaluated by the nitroblue tetrazolium assay, and the complexes catalyzed the dismutation of superoxide at pH 7.8 with IC 50 values of 0.35–1.25 μ M . The complexes have also been screened for their antibacterial activity against five pathogenic bacteria.