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Cytotoxic Activity of Fungal Strains Isolated from the Ascidian Eudistoma vannamei
Author(s) -
Montenegro Tasso G. C.,
Rodrigues Felipe A. R.,
Jimenez Paula C.,
Angelim Alysson L.,
Melo Vânia M. M.,
Rodrigues Filho Edson,
de Oliveira Maria da Conceição F.,
CostaLotufo Letícia V.
Publication year - 2012
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201100366
Subject(s) - chemistry , cytotoxic t cell , cytotoxicity , bioassay , fermentation , cell culture , fungus , microbiology and biotechnology , aspergillus , aspergillus fumigatus , biochemistry , biology , in vitro , botany , genetics
The cytotoxic activity at 50 μg/ml of extracts obtained from eleven fungal strains associated to Eudistoma vannamei , an endemic ascidian from Northeast Brazil, against two cell lines, i.e. , the HCT‐8 (colon cancer) and the MDA‐MB‐435 (melanoma) cell lines, was investigated. The most promising extract ( EV10 ) was obtained from a fungus identified as Aspergillus sp. by molecular analysis and was selected for bioassay‐guided isolation of its active principals. Large‐scale fermentation of EV10 in potato‐dextrose broth followed by chromatographic purification of the active extract from the liquid medium allowed the isolation of the isocoumarins mellein, cis ‐4‐hydroxymellein, and trans ‐4‐hydroxymellein, besides penicillic acid. All isolated compounds were tested for their cytotoxicity against the tumor cell lines MDA‐MB‐435 and HCT‐8 and revealed penicillic acid as the only cytotoxic compound (cell growth inhibitions >95%).