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Diverse Secondary Metabolites Produced by Marine‐Derived Fungus Nigrospora sp. MA75 on Various Culture Media
Author(s) -
Shang Zhuo,
Li XiaoMing,
Li ChunShun,
Wang BinGui
Publication year - 2012
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201100216
Subject(s) - griseofulvin , chemistry , fungus , fermentation , bioassay , plant use of endophytic fungi in defense , stereochemistry , botany , food science , biology , medicine , genetics , pathology
Bioassay‐guided isolation of a fungal strain Nigrospora sp. MA75, an endophytic fungus obtained from the marine semi‐mangrove plant Pongamia pinnata , which was fermented on three different culture media, resulted in the isolation and identification of seven known compounds, 2, 3 , and 5 – 9 , from a medium containing 3.5% NaCl, while a new compound, 2,3‐didehydro‐19 α ‐hydroxy‐14‐epicochlioquinone B ( 10 ) was obtained from the medium containing 3.5% NaI. In addition, two new griseofulvin derivatives, 6‐ O ‐desmethyldechlorogriseofulvin ( 1 ) and 6′‐hydroxygriseofulvin ( 4 ), were isolated and identified from the rice solid medium. Dechlorogriseofulvin ( 2 ) and griseofulvin ( 3 ) were the major components in fermentation extracts of all these culture media, while compounds 1 and 4, 5 and 6 , and 10 were only present in the extract of respective culture medium. The structures of these compounds were elucidated by detailed spectroscopic analysis, and the absolute configuration of 1 was determined by CD measurement. Compounds 9 and 10 exhibited antibacterial activities toward five tested bacterial strains, while compounds 5, 6 , and 8 selectively inhibited MRSA, E. coli , and S. epidermidis , and compound 3 showed moderate activity against V. mali and S. solani. Moreover, compound 10 potently inhibited the growth of MCF‐7, SW1990, and SMMC7721 tumor cell lines with IC 50 values of 4, 5, and 7 μg/ml, respectively.