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Haemolytic Activity and Immunological Adjuvant Effect of a New Steroidal Saponin from Allium ampeloprasum var. porrum
Author(s) -
Adão Camila Rodrigues,
Pereira da Silva Bernadete,
Tinoco Luzineide Wanderley,
Parente José Paz
Publication year - 2012
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201100005
Subject(s) - saponin , chemistry , adjuvant , allium , ovalbumin , in vitro , immune system , antigen , traditional medicine , stereochemistry , botany , biochemistry , immunology , biology , pathology , medicine , alternative medicine
Abstract A new steroidal saponin was isolated from the bulbs of Allium ampeloprasum L. var. porrum. On the basis of chemical evidence, comprehensive spectroscopic analyses, and comparison with known compounds, its structure was established as (3 β ,5 α ,6 β ,25 R )‐3‐{( O ‐ β ‐ D ‐glucopyranosyl‐(1→3)‐ β ‐ D ‐glucopyranosyl‐(1→2)‐ O ‐[ O ‐ β ‐ D ‐glucopyranosyl‐(1→3)]‐ O ‐ β ‐ D ‐glucopyranosyl‐(1→4)‐ β ‐ D ‐galactopyranosyl)oxy}‐6‐hydroxyspirostan‐2‐one ( 1 ). Results of the present study indicated that 1 exhibited haemolytic activity in the in vitro assays, and immunological adjuvant activity on the cellular immune response against ovalbumin antigen.