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Antioxidant Phenolic Compounds from the Stems of Entada phaseoloides
Author(s) -
Dong Yuqiong,
Shi Haiming,
Yang Haisha,
Peng Yunhua,
Wang Mengyue,
Li Xiaobo
Publication year - 2012
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201100002
Subject(s) - chemistry , antioxidant , abts , dpph , fractionation , fraction (chemistry) , superoxide , traditional medicine , chromatography , biochemistry , enzyme , medicine
The EtOH extract of the stems of Entada phaseoloides displayed potent antioxidant activity when assessed by the 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radical‐scavenging, reducing power, β ‐carotene‐bleaching, and superoxide radical‐scavenging analyses. Fractionation of the EtOH extracts showed that the AcOEt fraction is the most active, followed by the H 2 O fraction, while BuOH fraction was least active. Further activity‐guided fractionation studies on the active fractions resulted in the isolation of 22 compounds. The identities of these compounds were established based on extensive spectroscopic studies. Furthermore, the antioxidant activities of the isolated compounds were evaluated by using the above‐mentioned five assays. The results demonstrated that the EtOH extract of E. phaseoloides stems exhibits an excellent antioxidant activity and thus presents a great potential as a source of natural antioxidants.