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Chemical Composition and Antimicrobial Activity of the Essential Oil of Endemic Dalmatian Black Pine ( Pinus nigra ssp. dalmatica )
Author(s) -
Politeo Olivera,
Skocibusic Mirjana,
Maravic Ana,
Ruscic Mirko,
Milos Mladen
Publication year - 2011
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201000185
Subject(s) - antimicrobial , essential oil , sabinene , chemistry , chemical composition , botany , antibacterial activity , germacrene , pinene , bacteria , food science , composition (language) , traditional medicine , biology , limonene , organic chemistry , medicine , genetics , linguistics , philosophy
The chemical composition and the antimicrobial activity of the essential oil isolated from the needles of endemic Dalmatian black pine ( Pinus nigra ssp. dalmatica ) from Croatia were investigated. The chemical composition of the essential oil was determined by GC and GC/MS analyses, and the main compounds identified were α ‐pinene, β ‐pinene, germacrene D, and β ‐caryophyllene. Disc‐diffusion and broth‐microdilution assays were used for the in vitro antimicrobial screening. The Dalmatian black pine essential oil exhibited a great potential of antibacterial activity against Gram ‐positive bacteria ( MIC =0.03–0.50% ( v / v )) and a less pronounced activity against Gram ‐negative bacteria ( MIC =0.12–3.2% ( v / v )). The volatile compounds also inhibited the growth of all fungi tested, including yeast.