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Secolignans with Antiangiogenic Activities from Peperomia dindygulensis
Author(s) -
Lin MengGan,
Yu DeHong,
Wang QiWei,
Lu Qin,
Zhu WenJun,
Bai Fan,
Li GuiXiu,
Wang XiangWen,
Yang YiFang,
Qin XueMei,
Fang Chao,
Chen HongZhuan,
Yang GuoHong
Publication year - 2011
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201000123
Subject(s) - methylenedioxy , chemistry , umbilical vein , stereochemistry , hydroxymethyl , bioassay , human umbilical vein endothelial cell , in vitro , biochemistry , organic chemistry , biology , alkyl , halogen , genetics
Two new secolignans, peperomins G and H ( 1 and 2 , resp.), were isolated from the whole plant of Peperomia dindygulensis , together with five known secolignans, peperomin A ( 3 ), peperomin E ( 4 ), peperomin B ( 5 ), 2,3‐ trans ‐2‐methyl‐3‐{(3‐hydroxy‐4,5‐dimethoxyphenyl)[5‐methoxy‐3,4‐(methylenedioxy)phenyl]methyl}butyrolactone ( 6 ), 2,3‐ cis ‐2‐(hydroxymethyl)‐3‐{bis[5‐methoxy‐3,4‐(methylenedioxy)phenyl]methyl}butyrolactone ( 7 ). Their structures and configurations were elucidated by spectroscopic methods including 2D‐NMR techniques. Antiangiogenic effects of all compounds were evaluated using human umbilical vein endothelial cells (HUVEC) proliferation and tube‐formation tests, with compounds 4 and 5 being active in the bioassay. Compounds 4 and 5 induced obvious cell toxicity to HUVEC with IC 50 values of 1.64±0.19 and 8.44±0.4 μ M , respectively. Compounds 4 and 5 also exhibited significant HUVEC tube formation‐inhibiting activity with IC 50 values of 3.13±0.09 and 6.24±0.12 μ M , respectively.