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An Alternative Standard for Trolox ‐Equivalent Antioxidant‐Capacity Estimation Based on Thiol Antioxidants. Comparative 2,2′‐Azinobis[3‐ethylbenzothiazoline‐6‐sulfonic Acid] Decolorization and Rotational Viscometry Study Regarding Hyaluronan Degradation
Author(s) -
Hrabárová Eva,
Valachová Katarína,
Rapta Peter,
Šoltés Ladislav
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.201000019
Subject(s) - trolox , chemistry , sulfonic acid , radical , antioxidant , trolox equivalent antioxidant capacity , thiol , abts , organic chemistry , chromatography , nuclear chemistry , antioxidant capacity , dpph
Comparison of the effectiveness of antioxidant activity of three thiol compounds, D ‐penicillamine, reduced L ‐glutathione, and 1,4‐dithioerythritol, expressed as a radical‐scavenging capacity based on the two independent methods, namely a decolorization 2,2′‐azinobis[3‐ethylbenzothiazoline‐6‐sulfonic acid] assay and a rotational viscometry, is reported. Particular concern was focused on the testing of potential free‐radical scavenging effects of thiols against hyaluronan degradation, induced by hydroxyl radicals. A promising, solvent‐independent, antioxidative function of 1,4‐dithioerythritol, comparable to that of a standard compound, Trolox ® , was confirmed by the 2,2′‐azinobis[3‐ethylbenzothiazoline‐6‐sulfonic acid] assay. The new potential antioxidant 1,4‐dithioerythritol exhibited very good solubility in a variety of solvents ( e.g. , H 2 O, EtOH, and DMSO) and could be widely accepted and used as an effective antioxidant standard instead of a routinely used Trolox ® on 2,2′‐azinobis[3‐ethylbenzothiazoline‐6‐sulfonic acid] assay.