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Glycoalkaloid Profile in Potato Haploids Derived from Solanum tuberosum – S. bulbocastanum Somatic Hybrids
Author(s) -
Carputo Domenico,
Savarese Salvatore,
Andolfi Anna,
Aversano Riccardo,
Cimmino Alessio,
Frusciante Luigi,
Evidente Antonio
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200900391
Subject(s) - glycoalkaloid , solanum tuberosum , hybrid , ploidy , solanum , biology , stamen , genome , somatic cell , solanaceae , human health , botany , genetics , gene , pollen , medicine , environmental health
Cultivated and wild potato species synthesize a wide variety of steroidal glycoalkaloids (GA) that may affect either human health or biotic stress resistance. Therefore, GA composition must be a major criterion in the evaluation of breeding products when species genomes are merged and/or manipulated. This work reports the results of GA analysis performed on unique haploid (2 n =2 x =24) plants obtained from tetraploid (2 n =4 x =48) Solanum bulbocastanum – S. tuberosum hybrids through in vitro anther culture. Glycoalkaloids were extracted from tubers and analyzed by HPLC. Haploids generally showed the occurrence of parental GA. However, in several cases loss of parental GA and gain of new GA lacking in the parents was observed. It may be hypothesized that new GA profiles of our haploids is the result of either genetic recombination or combinatorial biochemistry events. To highlight differences between haploids and parents, soluble proteins and antioxidant activities were also determined. Both were always higher in haploids compared to their parents. The nature of the newly formed GAs will be further investigated, because they may represent new metabolites that can be used against pest and diseases, or are useful for human health.