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7,8‐Secolignans from Schisandra wilsoniana and Their Anti‐HIV‐1 Activities
Author(s) -
Zhang XingJie,
Yang GuangYu,
Wang RuiRui,
Pu JianXin,
Sun HanDong,
Xiao WeiLie,
Zheng YongTang
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200900367
Subject(s) - chemistry , peripheral blood mononuclear cell , human immunodeficiency virus (hiv) , syncytium , schisandra , stereochemistry , microbiology and biotechnology , virology , biochemistry , in vitro , cell , biology , medicine , pathology , alternative medicine , traditional chinese medicine
Two new 7,8‐secolignans, marphenols A and B ( 1 and 2 , resp.), together with a known related derivative, 7,8‐secoholostylone B ( 3 ), were isolated from the stems of Schisandra wilsoniana. The structures of 1 and 2 were elucidated by spectroscopic methods, including extensive 1D‐ and 2D‐NMR techniques. The anti‐HIV‐1 activities of 1 – 3 were evaluated. Compound 1 inhibited HIV‐1 IIIB ‐induced syncytia formation with an EC 50 value of 0.55 μg ml −1 . It reduced p24 antigen expression in acutely HIV‐1 IIIB ‐infected C8166 cells and primary isolate HIV‐1 TC‐2 ‐infected peripheral blood mononuclear cells (PBMCs), with EC 50 values of 3.34 and 0.52 μg ml −1 , respectively. It showed no effects on the HIV‐1 IIIB replication in chronically infected H9 cells as well as fusion inhibition.